报告题目：Nucleic acid recognitions: perspectives from site-directed spin labeling
报告人：Peter Qin 教授
美国南加州大学
报告时间：2019年3月13日（周三）下午4:00
报告地点：石先楼二楼学术报告厅
报告摘要：
In site-directed spin labeling (SDSL), a stable radical, usually a nitroxide, is covalently attached at a specific location within a macro-molecule, and structural and dynamic information at the labeling site is obtained via electron paramagnetic resonance (EPR) spectroscopy. My group pioneered the development of a family of nitroxide labels, called the R5-series, which can be attached in an efficient and cost-effective manner to a specific phosphorothioate backbone position at arbitrary DNA or RNA sequences. Using the R5-series, we have obtained unique information on nucleic acids and protein-nucleic acid complexes in bulk solutions and at the single-molecule level. I will present an overview of our work with a focus on studying sequence-dependent DNA deformation by the p53 tumor suppressor and investigating DNA unwinding by CRISPR-Cas.
